2.43
1,65
1,98
0,73
1,88
1,81
2.43
2.2 Standardne supstance korištene u kalibracijskoj krivulji relativne raspodjele molekularne mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti i oprema
23.2
21.4
22.2
16.1
22.3
20,8
23,9
27,5
Sveukupno, udio aminokiselina u Sustarovim proizvodima je veći nego u Zinproovim proizvodima.
Dio 8 Efekti upotrebe
Utjecaj različitih izvora elemenata u tragovima na proizvodne performanse i kvalitet jaja nosilja u kasnom periodu nošenja.
Proizvodni proces
Tehnologija ciljane helacije
Tehnologija emulgiranja smicanjem
Tehnologija prskanja i sušenja pod pritiskom
Tehnologija hlađenja i odvlaživanja
Napredna tehnologija kontrole okoline
Dodatak A: Metode za određivanje relativne raspodjele molekularne mase peptida
Usvajanje standarda: GB/T 22492-2008
1 Princip testiranja:
Određena je visokoefikasnom gel filtracijskom hromatografijom. To jest, korištenjem poroznog punila kao stacionarne faze, na osnovu razlike u relativnoj molekularnoj masi komponenti uzorka za razdvajanje, detektovane na peptidnoj vezi ultraljubičaste apsorpcijske talasne dužine od 220 nm, korištenjem namjenskog softvera za obradu podataka za određivanje distribucije relativne molekularne mase gel filtracijskom hromatografijom (tj. GPC softver), hromatogrami i njihovi podaci su obrađeni, izračunati da bi se dobila veličina relativne molekularne mase sojinog peptida i raspon distribucije.
2. Reagensi
Eksperimentalna voda treba da ispunjava specifikacije sekundarne vode u GB/T6682, a upotreba reagensa, osim posebnih odredbi, mora biti analitički čista.
2.1 Reagensi uključuju acetonitril (hromatografski čist), trifluorosirćetnu kiselinu (hromatografski čistu),
2.2 Standardne supstance korištene u kalibracijskoj krivulji relativne raspodjele molekularne mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti i oprema
3.1 Visokoučinkovita tečna hromatografija (HPLC): hromatografska radna stanica ili integrator sa UV detektorom i softverom za obradu podataka GPC-a.
3.2 Jedinica za vakuumsku filtraciju i degazaciju mobilne faze.
3.3 Elektronska vaga: graduirana vrijednost 0,000 1 g.
4 Koraka rada
4.1 Kromatografski uslovi i eksperimenti adaptacije sistema (referentni uslovi)
- 4.1.1 Kromatografska kolona: TSKgelG2000swxl300 mm×7,8 mm (unutrašnji prečnik) ili druge gel kolone istog tipa sa sličnim performansama pogodne za određivanje proteina i peptida.
- 4.1.2 Mobilna faza: Acetonitril + voda + trifluorosirćetna kiselina = 20 + 80 + 0,1.
- 4.1.3 Talasna dužina detekcije: 220 nm.
- 4.1.4 Brzina protoka: 0,5 mL/min.
- 4.1.5 Vrijeme detekcije: 30 minuta.
- 4.1.6 Volumen ubrizgavanja uzorka: 20 μL.
- 4.1.7 Temperatura kolone: sobna temperatura.
- 4.1.8 Da bi hromatografski sistem ispunio zahtjeve detekcije, propisano je da pod gore navedenim hromatografskim uslovima, efikasnost gel hromatografske kolone, tj. teorijski broj ploča (N), ne bude manja od 10000 izračunato na osnovu vrhova tripeptidnog standarda (Glicin-Glicin-Glicin).
- 4.2 Izrada standardnih krivulja relativne molekularne mase
- Gore navedeni rastvori standarda peptida s različitim relativnim molekulskim masama i masenom koncentracijom od 1 mg/mL pripremljeni su metodom usklađivanja mobilne faze, pomiješani u određenom omjeru, a zatim filtrirani kroz membranu organske faze s veličinom pora od 0,2 μm do 0,5 μm i ubrizgani u uzorak, nakon čega su dobiveni kromatogrami standarda. Kalibracijske krivulje relativne molekulske mase i njihove jednadžbe dobivene su crtanjem logaritma relativne molekulske mase u odnosu na vrijeme zadržavanja ili linearnom regresijom.
4.3 Obrada uzorka
Precizno izvagati 10 mg uzorka u odmjernoj tikvici od 10 mL, dodati malo mobilne faze, ultrazvučno mućkati 10 minuta, tako da se uzorak potpuno rastvori i pomiješa, razrijediti mobilnom fazom do vage, a zatim filtrirati kroz membranu organske faze s veličinom pora od 0,2 μm ~ 0,5 μm, a filtrat analizirati prema hromatografskim uvjetima u A.4.1.
- 5. Izračunavanje relativne raspodjele molekulskih masa
- Nakon analize rastvora uzorka pripremljenog u 4.3 pod hromatografskim uslovima iz 4.1, relativna molekularna masa uzorka i njen opseg distribucije mogu se dobiti zamjenom hromatografskih podataka uzorka u kalibracionu krivulju 4.2 pomoću GPC softvera za obradu podataka. Distribucija relativnih molekularnih masa različitih peptida može se izračunati metodom normalizacije površine vrha, prema formuli: X=A/A ukupno×100
- U formuli: X - Maseni udio relativne molekulske mase peptida u ukupnom peptidu u uzorku, %;
- A - Površina vrha relativne molekularne mase peptida;
- Ukupno A - zbir površina vrhova svakog peptida relativne molekulske mase, izračunat na jednu decimalu.
- 6 Ponovljivost
- Apsolutna razlika između dva nezavisna određivanja dobijena pod uslovima ponovljivosti ne smije prelaziti 15% aritmetičke sredine dva određivanja.
- Dodatak B: Metode za određivanje slobodnih aminokiselina
- Usvajanje standarda: Q/320205 KAVN05-2016
- 1.2 Reagensi i materijali
- Ledena sirćetna kiselina: analitički čista
- Perhlorna kiselina: 0,0500 mol/L
- Indikator: 0,1% kristal ljubičasti indikator (glacijalna sirćetna kiselina)
- 2. Određivanje slobodnih aminokiselina
Uzorci su sušeni na 80°C tokom 1 sata.
Uzorak stavite u suhu posudu da se prirodno ohladi na sobnu temperaturu ili na temperaturu pogodnu za korištenje.U suhu konusnu tikvicu od 250 mL odvažite približno 0,1 g uzorka (s tačnošću od 0,001 g).Brzo pređite na sljedeći korak kako biste spriječili da uzorak apsorbira vlagu iz okoline.Dodajte 25 mL glacijalne sirćetne kiseline i dobro miješajte ne duže od 5 minuta.Dodajte 2 kapi indikatora kristal ljubičaste bojeTitrirati sa 0,0500 mol/L (±0,001) standardnim titracionim rastvorom perhlorne kiseline dok rastvor ne promijeni boju iz ljubičaste u krajnju tačku.
Zabilježite količinu potrošenog standardnog rastvora.
- Istovremeno provedite slijepi test.
- 3. Izračun i rezultati
- Sadržaj slobodnih aminokiselina X u reagensu izražava se kao maseni udio (%) i izračunava se prema formuli: X = C × (V1-V0) × 0,1445/M × 100%, u formuli:
- C - Koncentracija standardnog rastvora perhlorne kiseline u molovima po litri (mol/L)
- V1 - Volumen korišten za titraciju uzoraka standardnim rastvorom perhlorne kiseline, u mililitrima (mL).
- Vo - Zapremina korištena za titraciju slijepe probe sa standardnim rastvorom perhlorne kiseline, u mililitrima (mL);
M - Masa uzorka, u gramima (g).
| 0,1445: Prosječna masa aminokiselina ekvivalentna 1,00 mL standardnog rastvora perhlorne kiseline [c (HClO4) = 1,000 mol / L]. | 4.2.3 Standardni titracijski rastvor cerijum sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripremljen prema GB/T601. | |
| Usvajanje standarda: Q/70920556 71-2024 | 1. Princip određivanja (Fe kao primjer) | Kompleksi aminokiselina i željeza imaju vrlo nisku topljivost u bezvodnom etanolu, a slobodni metalni ioni su topljivi u bezvodnom etanolu. Razlika u topljivosti između njih dvoje u bezvodnom etanolu korištena je za određivanje brzine helacije kompleksa aminokiselina i željeza. |
| U formuli: V1 - zapremina standardnog rastvora cerijum sulfata utrošenog za titraciju ispitivanog rastvora, mL; | Bezvodni etanol; ostatak je isti kao u klauzuli 4.5.2 u GB/T 27983-2011. | 3. Koraci analize |
| Uradite dva paralelna pokušaja. Izvažite 0,1 g uzorka sušenog na 103 ± 2 ℃ tokom 1 sata, sa tačnošću od 0,0001 g, dodajte 100 mL bezvodnog etanola da se rastvori, filtrirajte, ostatak filtera isperite sa 100 mL bezvodnog etanola najmanje tri puta, zatim prebacite ostatak u konusnu tikvicu od 250 mL, dodajte 10 mL rastvora sumporne kiseline prema klauzuli 4.5.3 u GB/T27983-2011, a zatim izvršite sljedeće korake prema klauzuli 4.5.3 „Zagrijte da se rastvori, a zatim ostavite da se ohladi“ u GB/T27983-2011. Istovremeno provedite slijepi test. | 4. Određivanje ukupnog sadržaja željeza | 4.1 Princip određivanja je isti kao u klauzuli 4.4.1 u GB/T 21996-2008. |
4.2. Reagensi i rastvori
| 4.2.1 Mješana kiselina: Dodajte 150 ml sumporne kiseline i 150 ml fosforne kiseline u 700 ml vode i dobro promiješajte. | 4.2.2 Indikatorski rastvor natrijum difenilamin sulfonata: 5 g/L, pripremljen prema GB/T603. | 4.2.3 Standardni titracijski rastvor cerijum sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripremljen prema GB/T601. | |
| 4.3 Koraci analize | Uradite dva paralelna pokušaja. Izvažite 0,1 g uzorka, tačno do 0,20001 g, stavite ga u konusnu tikvicu od 250 ml, dodajte 10 ml miješane kiseline, nakon rastvaranja dodajte 30 ml vode i 4 kapi indikatorskog rastvora natrijum dianilin sulfonata, a zatim izvršite sljedeće korake prema tački 4.4.2 u GB/T21996-2008. Istovremeno provedite i slijepu probu. | 4.4 Predstavljanje rezultata | Ukupni sadržaj željeza X1 u kompleksima željeza aminokiselina, izražen u masenom udjelu željeza, izračunat je prema formuli (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - standardni rastvor cerijum sulfata utrošen za titraciju rastvora slijepe probe, mL; | V0 - standardni rastvor cerijum sulfata utrošen za titraciju rastvora slijepe probe, mL; | C - Stvarna koncentracija standardnog rastvora cerijum sulfata, mol/L5. Izračunavanje sadržaja željeza u helatimaSadržaj željeza X2 u helatu, izražen kao maseni udio željeza, vrijednost izražena u %, izračunat je prema formuli: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| U formuli: V1 - zapremina standardnog rastvora cerijum sulfata utrošenog za titraciju ispitivanog rastvora, mL; | V2 - standardni rastvor cerijum sulfata utrošen za titraciju rastvora slijepe probe, mL;nom1 - Masa uzorka, g. Kao rezultat određivanja uzima se aritmetička sredina rezultata paralelnog određivanja, a apsolutna razlika rezultata paralelnog određivanja nije veća od 0,3%. | 0,05585 - masa željeza (fero-željeza) izražena u gramima, ekvivalentna 1,00 mL standardnog rastvora cerijum-sulfata C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1 - Masa uzorka, g. Kao rezultat određivanja uzima se aritmetička sredina rezultata paralelnog određivanja, a apsolutna razlika rezultata paralelnog određivanja nije veća od 0,3%. | 6. Izračunavanje stope helacijeBrzina helacije X3, vrijednost izražena u %, X3 = X2/X1 × 100Dodatak C: Metode za određivanje brzine kelacije Zinproa |
Usvajanje standarda: Q/320205 KAVNO7-2016
1. Reagensi i materijali
a) Glacijalna sirćetna kiselina: analitički čista; b) Perhlorna kiselina: 0,0500 mol/L; c) Indikator: 0,1% kristal ljubičasti indikator (glacijalna sirćetna kiselina)
2. Određivanje slobodnih aminokiselina
2.1 Uzorci su sušeni na 80°C tokom 1 sata.
2.2 Stavite uzorak u suhu posudu da se prirodno ohladi na sobnu temperaturu ili da se ohladi na upotrebljivu temperaturu.
2.3 Odvažite približno 0,1 g uzorka (tačno do 0,001 g) u suhu konusnu tikvicu od 250 mL.
2.4 Brzo prijeđite na sljedeći korak kako biste spriječili da uzorak apsorbira vlagu iz okoline.
2.5 Dodajte 25 mL glacijalne sirćetne kiseline i dobro miješajte ne duže od 5 minuta.
2.6 Dodajte 2 kapi indikatora kristal violet.
2.7 Titrirajte sa standardnim titracionim rastvorom perhlorne kiseline koncentracije 0,0500 mol/L (±0,001) dok se rastvor ne promijeni iz ljubičaste u zelenu boju tokom 15 sekundi bez promjene boje kao krajnje tačke.
2.8 Zabilježite količinu utrošenog standardnog rastvora.
2.9 Slijepu probu provedite istovremeno.
- 3. Izračun i rezultati
- Katalonski
- Physicochemical parameters
V1 - Volumen korišten za titraciju uzoraka standardnim rastvorom perhlorne kiseline, u mililitrima (mL).
Vo - Zapremina korištena za titraciju slijepe probe sa standardnim rastvorom perhlorne kiseline, u mililitrima (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adresa: Br. 147 Qingpu Road, grad Shouan, okrug Pujiang, grad Chengdu, provincija Sečuan, Kina
Telefon: 86-18880477902
Proizvodi
Neorganski minerali u tragovima
- Organski minerali u tragovima
- Svahili
- Prilagođena usluga
- Brze veze
Profil kompanije
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gudžaratski | Kliknite za upit | © Autorsko pravo - 2010-2025: Sva prava pridržana. | Mapa sajta NAJBOLJA PRETRAGA Telefon |
| Tel. | 86-18880477902 | Javanski | E-pošta |
| 8618880477902 | kineski | Francuski | |
| Bird | kineski | Francuski | njemački Španski |
| Aquatic animals | Japanski | Korejski | arapski Grčki |
| turski | Italijanski | ||
| Ruminant animal g/head day | January 0.75 | Indonezijski Afrikanerski švedski |
Poljski
- baskijski
- Katalonski
- Physicochemical parameters
Hindski
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
bugarski
- Cebuanski
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- hrvatski
holandski
| Application object | Urdu Vijetnamski | Content in full-value feed (mg/kg) | Efficacy |
| Gudžaratski | haićanski | Hausa | kinjaruanda Hmong Mađarski |
| Piglets and fattening pigs | Igbo | Javanski | Kannada Kmerski Kurdski |
| Kirgizi | Latinski | ||
| Bird | 300～400 | 45～60 | Makedonski Malajski Malajalamski |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norveški
- Paštunski
- Appearance: brownish-yellow granules
- Physicochemical parameters
Srpski
Sesoto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Svahili
Tadžikistanski
Tamilski
Telugu
Tajlandski
| Application object | Urdu Vijetnamski | Content in full-value feed (mg/kg) | Efficacy |
| Jidiš | Joruba | Zulu | kinjaruanda Oriya Turkmenski |
| Ujgur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
